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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 4, 2012, pp. 1-7
Bioline Code: ej12030
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 4, 2012, pp. 1-7

 en Callus induction and plant regeneration of Ulex europaeus check for this species in other resources
Ramírez, Ingrid; Dorta, Fernando; Cuadros-Inostroza, Álvaro & Peña-Cortés, Hugo

Abstract

Abstract A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus check for this species in other resources . Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.

Keywords
antioxidants; callus induction; Fabaceae; gorse; plant regeneration; Ulex europaeus

 
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