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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 4, 2012, pp. 1-8
Bioline Code: ej12031
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 4, 2012, pp. 1-8

 en Production of phenolic metabolites by Deschampsia antarctica check for this species in other resources shoots using UV-B treatments during cultivation in a photobioreactor
Sequeida, Álvaro; Tapia, Eduardo; Ortega, Marcelo; Zamora, Pablo; Castro, Álvaro; Montes, Christian; Zúñiga, Gustavo E. & Prieto, Humberto

Abstract

Abstract Deschampsia antarctica check for this species in other resources (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA.

Keywords
Deschampsia antarctica; phenolic compounds; temporary immersion bioreactor; UV-B elicitation

 
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