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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 4, 2012, pp. 1-11
Bioline Code: ej12034
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 4, 2012, pp. 1-11

 en Effect of extrinsic and intrinsic parameters on inulinase production by Aspergillus niger check for this species in other resources ATCC 20611
Dinarvand, Mojdeh; Ariff, Arbakariya B.; Moeini, Hassan; Masomian, Malihe; Mousavi, Seyed Sadegh; Nahavandi, Reza & Mustafa, Shuhaimi

Abstract


Background: Inulinase is a versatile enzyme from glycoside hydrolase family which targets the β-2, 1 linkage of fructopolymers. In the present study, the effect of medium composition and culture conditions on inulinase production by Aspergillus niger check for this species in other resources ATCC 20611 was investigated in shake-flasks.
Results: The highest extracellular inulinase (3199 U/ ml) was obtained in the presence of 25% (w/v) sucrose, 0.5% (w/v) meat extract, 1.5% (w/v) NaNO3 and 2.5 mM (v/v) Zn2+, at initial pH of 6.5, temperature 35ºC and 6% (v/v) of spores suspension in the agitation speed of 100 rpm. Surfactants showed an inhibitory effect on enzyme production. The optimum temperature for inulinase activity was found to be 50ºC. TLC analysis showed the presence of both exo- and endo-inulinase.
Conclusion: Sucrose, Zn2+, and aeration were found to be the most effective elements in inulinase production by A. niger ATCC 20611. TLC analysis also showed that the crude enzyme contained both endo and exoinulinases. The strain is suggested as a potential candidate for industrial enzymatic production of fructose from inulin.

Keywords
Aspergillus niger ATCC 20611; culture optimization; inulinase

 
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