Background: Plant cell suspension culture of
Vitis vinifera
is a promising technology for investigating
different factors that are able to induce and/or modify stilbenes biosynthesis. Jasmonates have been
reported to play an important role in a signal transduction pathway that regulates defence responses as
well as the production of secondary metabolites. In this study, 2, 3-dihydroxypropyl jasmonate (DHPJA)
was used to investigate its effect on stimulating
trans-resveratrol (
t-R) accumulation and the plant
defence responses in
Vitis vinifera cv. Kyoho cell suspension cultures for the first time.
Results: It
demonstrated that DHPJA had superior effects on stilbenoids accumulation over methyl jasmonate
(MeJA). The optimal condition was 150 μM DHPJA added on day 15 of cultivation period, with the
highest level of
t-R accumulation which was increased 1.8-fold and 1.3-fold compared with the control
and 150 μM MeJA respectively. DHPJA induced stronger plant defence responses, including oxidative
burst and activation of L-phenylalanine ammonia lyase (PAL) than MeJA. H
2O
2 generation induced by
DHPJA played a significant role in enhancing
t-R accumulation. Adding a specific inhibitor of H
2O
2
signalling pathway inhibited DHPJA-induced
t-R accumulation, but had no effects on DHPJA-induced
other metabolites accumulation, which resulted in regulations of product diversity.
Conclusions: This
study demonstrated that DHPJA was an efficient elicitor to enhance
t-R accumulation by activating
stronger oxidative burst, and H
2O
2 signalling pathway could regulate product diversity in DHPJA-induced
V. vinifera cv. Kyoho cell suspension cultures.
