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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 15, No. 6, 2012
Bioline Code: ej12060
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 15, No. 6, 2012

 en Optimization of β-carotene production by a newly isolated Serratia marcescens check for this species in other resources strain
Wang, Bohua; Lin, Liping; Lu, Lei & Chen, Weiping


Abstract β-carotene is a commonly used food colorant. In this work, a novel β-carotene producing strain, Serratia marcescens check for this species in other resources RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of β-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for β-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe2+, pH 6.0 and 30ºC. Under the optimal conditions, the yield of β-carotene achieved 2.45 μg/mL.

β-carotene; optimization; pigment; Serratia marcescens

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