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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 16, No. 2, 2013, pp. 1-12
Bioline Code: ej13010
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 16, No. 2, 2013, pp. 1-12

 en DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis check for this species in other resources
Pomponio, María F.; Torales, Susana; Gallo, Leonardo A.; Pastorino, Mario J.; Marchelli, Paula; Cervera, María Teresa & Poltri, Susana Marcucci

Abstract


Background: Austrocedrus chilensis check for this species in other resources (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress.
Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversity value (π = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection.
Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species.

Keywords
adaptive variation; balancing selection; Patagonian cypress; single nucleotide polymorphisms (SNPs)

 
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