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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 16, No. 2, 2013, pp. 1-13
Bioline Code: ej13012
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 16, No. 2, 2013, pp. 1-13

 en Evaluating Colombian Clostridium check for this species in other resources spp. strains’ hydrogen production using glycerol as substrate
Bernal, Mauricio; Tinoco, Lizeth K.; Torres, Luz; Malagón-Romero, Dionisio & Montoya, Dolly

Abstract


Background: The ability for hydrogen production of 13 native strains of Clostridium check for this species in other resources spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00).
Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1, productivity 0.3167 mg H2.h-1 l-1 culture mediumand yield 0.1962 mol H2.mol-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l-1.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R2 = 0.95 for biomass substrate, R2 = 0.77 hydrogen production).
Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible.

Keywords
anaerobic fermentation; biofuel; Clostridium spp.; glycerol; hydrogen; mathematical model

 
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