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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 16, No. 3, 2013
Bioline Code: ej13024
Full paper language: English
Document type: Review Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 16, No. 3, 2013

 en Metabolic engineering: Use of system-level approaches and application to fuel production in Escherichia coli check for this species in other resources
González, Ramón

Abstract

Metabolic engineering was formally defined more than two decades ago (Bailey, 1991) and it is now an established discipline. Metabolic engineering is generally defined as the directed improvement of product formation or cellular properties through the modification of specific biochemical reactions or the introduction of new ones with the use of recombinant DNA technology (Bailey, 1991; Stephanopoulos et al. 1998). Therefore, the analysis and engineering/synthesis of metabolic pathways is of central importance to metabolic engineering. The analytical part uses a number of experimental and modeling techniques for the systematic study of cellular responses (in terms of RNA, protein and metabolite levels, metabolic fluxes, etc.) to genetic and environmental perturbations. This facilitates a rational design of metabolic modifications, which are implemented using recombinant DNA technology. Both, the analysis and the synthesis of metabolic pathways will be covered in this review. Recent efforts on the engineering of fermentative and biosynthetic pathways for biofuel production in Escherichia coli check for this species in other resources , as well as those enabling the utilization of novel feedstocks, will be highlighted.

Keywords
biofuels; Escherichia coli; metabolic engineering; systems biology

 
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