Background: Interleukin-22 (IL-22) plays an important role in the regulation of immune responses.
However, little is known about its function or structure in fish.
Results: The IL-22 gene was first cloned from So-iny mullet (
Liza haematocheila
), one of commercially
important fish species in China. Then, 3-D structure model of the mullet IL-22 was constructed by
comparative modeling method using human IL-22 (1M4R) as template, and a 5 ns molecular dynamics
(MD) was studied. The open reading frame (ORF) of mullet IL-22 cDNA was 555 bp, encoding 184
amino acids. The mullet IL-22 shared higher identities with the other fish IL-22 homologs and
possessed a conserved IL-10 signature motif at its C-terminal. The mullet IL-22 model possessed six
conserved helix structure. PROCHECK, SAVES and Molprobity server analysis confirmed that this
model threaded well with human IL-22. Strikingly, analysis with CastP, cons-PPISP server suggested
that the cysteines in mullet IL-22 might not be involved in the forming of disulfide bond for structural
stabilization, but related to protein-protein interactions.
Conclusions: The structure of IL-22 in So-iny mullet (
Liza haematocheila) was constructed using
comparative modeling method which provide more information for studying the function of fish IL-22.
