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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 16, No. 4, 2014
Bioline Code: ej13034
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 16, No. 4, 2014

 en Prodigiosin found in Serratia marcescens check for this species in other resources y2 initiates phototoxicity in the cytomembrane
Wang, Fei; Luo, Hailan; Song, Guohua; Liu, Chang; Wang, Jianguo; Xu, Jingliang; Su, Xiuhong & Ma, Xu Yuan

Abstract

Background: Light can be absorbed by bacterial pigment and affects its growth. Prodigiosin is a red pigment found in various bacterial species. The purpose of this study was to investigate the impacts of light on prodigiosin production, biomass formation, and membrane integrity of Serratia marcescens check for this species in other resources y2.
Results: S. marcescens y2 grew better and produced more intracellular prodigiosin in darkness than in illumination. The pigment leakage ratio from cells was detected more in light than in darkness conditions. Ethidium bromide uptake assay could visually prove the prodigiosin-related loss of membrane integrity under illumination. A higher concentration of malondialdehyde (MDA) was detected in light-treated culture than in darkness. Tests of different light treatments (red, yellow, blue and green) showed that the maximum extracellular pigment and the minimum biomass formation and intracellular pigment were obtained in green light.
Conclusions: Prodigiosin could absorb light, and then initiate phototoxicity damage of the cytomembrane.

Keywords
ethidium bromide; homogeneous light; malondialdehyde; red pigment

 
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