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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 16, No. 4, 2014
Bioline Code: ej13042
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 16, No. 4, 2014

 en Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus check for this species in other resources in submerged fermentations
Díaz, Rubén; Téllez-Téllez, Maura; Sánchez, Carmen; Bibbins-Martínez, Martha D.; Díaz- Godínez, Gerardo & Jorge, Soriano-Santos

Abstract

Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus check for this species in other resources grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these cultures were also studied.
Results: The highest laccases activity was obtained in cultures grown at initial pH of 4.5 and the lowest in cultures grown at initial pH of 8.5. Isoenzyme profiles were different in all the cases. Lacc1, Lacc4, Lacc6 and Lacc10 were expressed in all the cultures.
Conclusions: The initial pH of the growing medium is an important factor for regulating the expression of laccase genes, having an effect on the activity and on the laccase isoenzymes number produced by P. ostreatus in SmF. This is the first report on the influence of different initial pH values of the growing medium on the laccases activity, laccase isoenzymes number and laccases expression profiles of P. ostreatus grown in submerged fermentation.

Keywords
laccases; Pleurotus ostreatus; submerged fermentation; zymogram

 
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