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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 16, No. 5, 2013
Bioline Code: ej13046
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 16, No. 5, 2013

 en Production of enzymes from Lichtheimia ramosa check for this species in other resources using Brazilian savannah fruit wastes as substrate on solid state bioprocessess
de Andrade Silva, Cinthia Aparecida; Lacerda, Maria Priscila Franco; Leite, Rodrigo Simões Ribeiro & Fonseca, Gustavo Graciano

Abstract

Background: Enzyme production by solid state bioprocess (SSB) using residues as substrate for microorganisms is an alternative for costs reduction and to avoid their disposal into environment. The aim of this work was to evaluate the physiology of the fungus Lichtheimia ramosa check for this species in other resources in terms of microbial growth and production of amylases, β-glucosidases, carboxymethylcellulase (CMCase), and xylanases, via SSB, utilizing wastes of the Brazilian savannah fruits bocaiuva ( Acrocomia aculeata check for this species in other resources ), guavira ( Campomanesia pubescens) check for this species in other resources and pequi ( Caryocar brasiliense check for this species in other resources ) as substrate at different temperatures (25, 30, and 35oC) during 168 hrs.
Results: Samples were taken every 24 hrs, which resulted in 8-points kinetic experiments to determine microbiological and enzymatic contents. The best substrate for β-glucosidase activity was pequi waste after 48 hrs at 30oC (0.061 U/mL). For amylase activity, bocaiuva presented itself as the best substrate after 96 hrs at 30oC (0.925 U/mL). CMCase activity was higher in guavira waste after 96 hrs at 35oC (0.787 U/mL). However, the activity was more expressive for xylanase in substrate composed of bocaiuva residue after 144 hrs at 35oC (1.802 U/mL).
Conclusions: It was concluded that best growth condition for L. ramosa is at 35oC for all substrates and that xylanase is the enzyme with more potential in SSB, considering the studied Brazilian savannah fruit wastes.

Keywords
amylase; β-glucosidase; CMCase; Lichtheimia ramosa; xylanase

 
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