Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 17, No. 1, 2014, pp. 34-38
Bioline Code: ej14006
Full paper language: English
Document type: Research Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 17, No. 1, 2014, pp. 34-38
© Copyright 2014 - Electronic Journal of Biotechnology
Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988|
Rufino, Raquel Diniz; de Luna, Juliana Moura; de Campos Takaki, Galba Maria & Sarubbo, Leonie Asfora
Background: Biotechnological processes are costly, especially for the production of biosurfactants. The successful
production of a biosurfactant is dependent on the development of processes using low cost raw materials.
Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the
properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have
Results: The yeast was grown for 72 h to determine the kinetics of growth and production. The surface tension of
the cell-free broth was reduced from 55 to 25 mN/m. The yield of biosurfactant was 8.0 g/l with a CMC of 0.03%.
The biosurfactant was characterized as an anionic lipopeptide composed of 50% protein, 20% lipids, and 8% of
Conclusions: The isolated biosurfactant showed no toxicity against different vegetable seeds: Brassica oleracea,
Solanum gilo and Lactuca sativa L. and the micro-crustacean Artemia salina. The properties of the biosurfactant
produced suggest its potential application in industries that require the use of effective compounds at low cost.
Biosurfactant; Candida lipolytica; Lipopeptide; Surface tension; Toxicity
Alternative site location: http://www.ejbiotechnology.info