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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 2, 2014, pp. 102-106
Bioline Code: ej14017
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 2, 2014, pp. 102-106

 en Assessment of somaclonal variation in somatic embryo-derived plants of yacon [ Smallanthus sonchifolius check for this species in other resources (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry
Viehmannova, Iva; Bortlova, Zuzana; Vitamvas, Jan; Cepkova, Petra Hlasna; Eliasova, Katerina; Svobodova, Eva & Travnickova, Martina

Abstract

Background: Yacon ( Smallanthus sonchifolius check for this species in other resources ) is a root crop native to the Andean region. Low sexual reproductive capacity is amajor constraint facing the genetic breeding of this crop. Biotechnological techniques offer alternative ways to widen genetic variability. We investigated somaclonal variation in regenerants of yacon derived from in vitro somatic embryogenesis using simple sequence repeat (ISSR) analysis and flow cytometry.
Results: Twenty tested ISSR primers provided a total of 7848 bands in 60 in vitro regenerants and control plant. The number of bands for each primer varied from3 to 10, and an average of 6.95 bands was obtained per ISSR primer. Eight primers were polymorphic and generated 10 polymorphic bands with 7.19% mean polymorphism. ISSR analysis revealed genetic variability in 6 plants under study. These regenerants had Jaccard's distances 0.104, 0.020, 0.040, 0.106, 0.163 and 0.040. Flow cytometric analysis did not reveal changes of relative nuclear DNA content in regenerants suggesting that the plants obtained via somatic embryogenesis had maintained stable octoploid levels.
Conclusions: Our findings show that indirect somatic embryogenesis could be used in yacon improvement to widen genetic variability, especially when low sexual reproductive capacity hinders classical ways of breeding

Keywords
DNA polymorphism; Molecular markers; Ploidy level; Proembryogenic callus

 
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