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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 3, 2014, pp. 107-113
Bioline Code: ej14018
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 3, 2014, pp. 107-113

 en Callus culture development of two varieties of Tagetes erecta check for this species in other resources and carotenoid production
Benítez-García, Israel; Vanegas-Espinoza, Pablo Emilio; Meléndez-Martínez, Antonio J.; Heredia, Francisco J.; Paredes-López, Octavio & Villar-Martínez, Alma Angélica Del

Abstract

Background: The properties of natural pigments, such as antioxidants, functional,medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poultry products (manly marigold flower extracts). Carotenoids such as β-carotene, β-criptoxanthin and lutein are very attractive as natural food colorants due to their antioxidant and pro-vitamin activities which provide additional value to the target products. Marigold ( Tagetes erecta check for this species in other resources ) is an Asteraceous ornamental plant native to Mexico, and it is also important as a carotenoid source for industrial and medicinal purposes but nowadays its production is destined mainly for ornamental purposes.
Results: Friable callus of T. erecta yellow flower (YF) and white flower (WF) varieties was induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 9.0 μM 4-dichlorophenoxyacetic acid (2,4-D) and 8.8 μM benzyladenine (BA). Calluses developed from both varieties were different in pigmentation. Extract characterization from callus cultures was carried out by high-performance liquid chromatography (HPLC). This analytical process detected several carotenoids; the main pigments in extracts from YF callus were lutein and zeaxanthin, whereas in the extracts of the WF callus the main pigmentswere lutein, zeaxanthin, β-cryptoxanthin and β-carotene. Callus cultures of T. erecta accumulated pigments even after several rounds of subculture.
Conclusions: WF callus appeared to be a suitable candidate as a source of different carotenoids, and tested varieties could represent an alternative for further studies about in vitro pigment production.

Keywords
2,4-D; BA; Cell development; Lutein; Marigold

 
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