A biosurfactant produced by Pseudomonas aeruginosa
cultivated in a low-cost medium formulated
with 2.5% vegetable oil refinery residue and 2.5% corn steep liquor and distilled water was employed to stabilize
silver nanoparticles in the liquid phase. The particleswere initially synthesized using NaBH4
as reducing agent in
biosurfactant reverse micelles and were extracted from the micellar solution to disperse in heptane.
A silver particle size in the range of 1.13 nmwas observed. The UV–vis absorption spectra proposed that
silver nanoparticles could be formed in the reverse micelles and relatively stabilized for at least 3 months without
passivator addition. The Transmission Electron Microscope (TEM) shows that the silver nanoparticles are of
spherical form and relatively uniform.
This process provided a simpler route for nanoparticle synthesis compared to existing systems using
whole organisms or partially purified biological extracts, showing that the low-cost biosurfactant can be used for
nanoparticle synthesis as a non-toxic and biodegradable stabilizing agent.