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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 4, 2014, pp. 156-161
Bioline Code: ej14026
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 4, 2014, pp. 156-161

 en Effects of oligosaccharides from endophytic Fusarium oxysporum check for this species in other resources Dzf17 on activities of defense-related enzymes in Dioscorea zingiberensis check for this species in other resources suspension cell and seedling cultures
Li, Peiqin; Luo, Haiyu; Meng, Jiajia; Sun, Weibo; Wang, Xiaohan; Lu, Shiqiong; Peng, Youliang & Zhou, Ligang

Abstract

Background: Three oligosaccharides (EOS, WOS and SOS) were respectively prepared from the corresponding polysaccharides, namely exopolysaccharide (EPS), water-extracted mycelial polysaccharide (WPS) and sodium hydroxide-extracted mycelial polysaccharides (SPS) from the endophytic fungus Fusarium oxysporum check for this species in other resources Dzf17. In this study, the effects of EOS,WOS and SOS on the activities of the defense-related enzymes, namely phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD) in its host plant Dioscorea zingiberensis check for this species in other resources cultures were investigated.
Results: For the suspension cell cultures of D. zingiberensis, the highest PAL activity was induced by 0.5 mg/mL of WOS at 48 h after treatment, which was 4.55-fold as that of control. Both PPO and POD activities were increased to themaximumvalues by 0.25 mg/mL ofWOS at 48 h after treatment,whichwere respectively 3.74 and 3.45-fold as those of control. For the seedling cultures, the highest PAL activity was elicited by 2.5 mg/mL of EOS at 48 h after treatment, which was 3.62-fold as that of control. Both PPO and POD reached theirmaximum values treatedwith 2.5 mg/mL ofWOS at 48 h after treatment, whichwere 4.61 and 4.19-fold as those of control, separately.
Conclusions: Both EOS and WOS significantly increased the activities of PAL, PPO and POD in the suspension cell and seedling cultures of D. zingiberensis. The results suggested that the oligosaccharides from the endophytic fungus F. oxysporum Dzf17 may be related to the activation and enhancement of the defensivemechanisms of D. zingiberensis suspension cell and seedling cultures.

Keywords
Endophytic fungus; Phenylalanine ammonia lyase; Peroxidase; Polyphenoloxidase; Polysaccharide

 
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