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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 4, 2014, pp. 162-167
Bioline Code: ej14027
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 4, 2014, pp. 162-167

 en Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
Fu, Changzhen; Wang, Hong; Li, Yaokun; Wang, Jiali; Cheng, Gong; Wang, Hongbao; Yang, Wucai & Zan, Linsen

Abstract

Background: CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acidmetabolic pathway and intracellular signal transduction in eukaryotic cells.
Results: In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its associationwith bodymeasurement andmeat quality traits of Qinchuan cattle. Blood samples were obtained from 638 Qinchuan cattle aged from 18 to 24 months. DNA sequencing and PCR-restriction fragment length polymorphism (RFLP) were used to find CDIPT gene single nucleotide polymorphism (SNP). Three SNPs g.244TN>C (NCBI: rs42069760), g.1496GN>A and g.1514GN>A were found in this study. g.244TN>C located at 5′untranslated region (5′UTR) of exon 1 showed three genotypes: TT, TC and CC. g.1496GN>A and g.1514GN>A detected the first time were located in intron 3 and showed the same genotypes: GG, GA and AA.
Conclusions: Analysis results showed that these three SNPswere significantly associated with body measurement traits (BMTs) andmeat quality traits (MQTs).We suggested that CDIPT genemay have potential effects on BMTs and MQTs and can be used for marker-assisted selection.

Keywords
Body measurement traits; Gene expression; Meat quality traits; PCR-RFLP; SNP

 
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