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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 5, 2014, pp. 204-210
Bioline Code: ej14034
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 5, 2014, pp. 204-210

 en Prolyl endopeptidase — Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus check for this species in other resources
Brzozowski, Bartosz & Lewandowska, Małgorzata

Abstract

Background: Lactic acid bacteria are able to reduce the immunoreactivity of proteins of cereal grains duringwheat dough fermentation ormay be a source of proteolytic preparations added during bread making. The key enzyme in prolamin degradation is prolyl endopeptidase. This studywas aimed at optimizing the composition of a culture medium and culture conditions that would enhance the synthesis of intracellular prolyl endopeptidase (PEP) by Lactobacillus acidophilus check for this species in other resources 5e2.
Results: The application of Plackett–Burman screening plans enabled demonstrating that the concentration of a nitrogen source in the culture and the initial pH value of the culture medium were significant for PEP synthesis. Further optimization conducted with the method of central composite designs (CCD) confirmed both the linear and square impact of nitrogen concentration and initial pH value of the culture medium on PEP production. In turn, the response surface method (RSM) allowed determining the optimal nitrogen concentration and pH value at 26.88 g/l and pH 4.85, respectively.
Conclusions: Validation of the resultant model enabled over 3-fold increase in the quantity of the synthesized enzyme.

Keywords
Central composite design; Plackett–Burman design; Response surface methodology

 
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