Background: Spermatogonial stem cells (SSCs) are important for the production of interspecies germ line
chimeras. The interspecies germ cell transfer technique has been suggested as a way to conserve endangered
birds. Our objective was to develop a technique for restoring endangered birds by developing interspecies
germ line chimeras between pheasant (
Phasianus colchicus
) and chicken (
Gallus gallus
) with SSCs.
Results: SSCs were isolated fromthe surgically removed testis of a pheasant. Growth conditions for pheasant SSCs
were established by co-culturing STO (SIM mouse embryo-derived thioguanine and ouabain resistant) cells and
pheasant SSCs. The colony-forming cells divided and proliferated stably to yield an established SSC line. Pheasant
SSCs showed strong reactivity for GDNF family receptor alpha1 (
GFRα1) marker. Finally, production of germline
chimeras was attempted by transferring pheasant SSCs into recipient embryos. Although final embryo survival
was 5.6% (20/354), the initial survival rate was 88% (312/354). To measure the percent transfer of donor SSC
to gonads, the pheasant SSCs were labeled with PKH 26 fluorescent dye. We observed 30% donor cells and
9.48% c-kit/CD117-positive cells in the gonads of recipient chickens. Donor SSCs were thus stably engrafted in
the recipient gonads.
Conclusions: This study showed that SSCs can be used as a tool for the conservation of endangered birds and the
production of germline chimeras. Our findings yield insights into howwe may use the pheasant spermatogonial
stem cell line for efficient production of interspecies germ line chimeras and ultimately, to the restoration of
endangered birds.
