Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 17, No. 5, 2014, pp. 224-229
Bioline Code: ej14037
Full paper language: English
Document type: Research Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 17, No. 5, 2014, pp. 224-229
© Copyright 2014 - Electronic Journal of Biotechnology
Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts|
Li, Xinxin; Wang, Jiwen; Liu, Hehe; Wang, Haohan; Sun, Lingli; Yang, Chao; Li, Liang & Zheng, Yi
Background: Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore
the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression
vector was constructed and identified. The biological activities of this vector were analyzed by transfecting
pEGFP-FST into cultured duck myoblasts using Lipofectamine™ 2000 and subsequently determining the mRNA
expression profiles of FST and myostatin (MSTN).
Results: The duck pEGFP-FST vector was successfully constructed and was confirmed to have high
liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with
pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in these cells
significantly inhibited the mRNA expression of MSTN (a target gene that is negatively regulated by FST).
Conclusions: The duck pEGFP-FST vector has been constructed successfully and exhibits biological activity
by promoting myoblast proliferation and differentiation in vitro.
Follistatin; Muscle hypertrophy; Overexpression; Transfection efficiency
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