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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 5, 2014, pp. 224-229
Bioline Code: ej14037
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 5, 2014, pp. 224-229

 en Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts
Li, Xinxin; Wang, Jiwen; Liu, Hehe; Wang, Haohan; Sun, Lingli; Yang, Chao; Li, Liang & Zheng, Yi

Abstract

Background: Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine™ 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN).
Results: The duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in these cells significantly inhibited the mRNA expression of MSTN (a target gene that is negatively regulated by FST).
Conclusions: The duck pEGFP-FST vector has been constructed successfully and exhibits biological activity by promoting myoblast proliferation and differentiation in vitro.

Keywords
Follistatin; Muscle hypertrophy; Overexpression; Transfection efficiency

 
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