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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 6, 2014, pp. 251-261
Bioline Code: ej14041
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 6, 2014, pp. 251-261

 en Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation
Ding, Fang; Yuan, Xin; Li, Qingqing; Sun, Wenqiang; Gan, Chao; He, Hua; Song, Chenling & Wang, Jiwen

Abstract

Background: Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL),which has been cloned from several species: Gallus gallus check for this species in other resources , Mus musculus check for this species in other resources , Homo sapiens check for this species in other resources , but not from Anas platyrhynchos check for this species in other resources . The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation.
Results: Wehave isolated a 7654 bp fragment fromPeking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05). However, 1000 μM treatment of oleic acid exhibited lipotoxic effect on cell viability (P < 0.05). In addition, during the first 24 h of duck adipocyte differentiation FAS was induced; however, after 24 h its expression level declined (P < 0.05).
Conclusion: We have successfully cloned and characterized Peking duck FAS. FAS was induced during adipocyte differentiation and by oleic acid treatment. These findings suggest that Peking duck FAS plays a similar role to mammalian FAS during adipocyte differentiation.

Keywords
Expression pattern; FAS; Oleic acid; Peking duck

 
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