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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 6, 2014, pp. 262-267
Bioline Code: ej14042
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 17, No. 6, 2014, pp. 262-267

 en Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
Yang, Weiping; Meng, Fanxu; Peng, Jiayin; Han, Peng; Fang, Fang; Ma, Li & Cao, Binyun


Background: The Tibetan pig is a pig breedwith excellent grazing characteristics indigenous to the Qinghai–Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig.
Results: A cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis; it was designated Bacillus subtilis check for this species in other resources BY-2. Examination of its growth characteristics showed that its growth curve entered the logarithmic phase after 8–12 h and the stable growth phase being between 20 and 40 h. The best carbon source for fermentation was 1% corn flour, while 2% peptone and yeast powder compound were the best nitrogen sources. The initial pH during fermentation was 5.5, with 4% inoculum, resulting in a high and stable amount of enzyme in 24–48 h.
Conclusions: The isolated BY-2 strain rapidly grew and produced cellulase.We believe that BY-2 cellulase can help overcome the shortage of endogenous animal cellulase, improve the utilization rate of roughage, and provide strain sources for research on porcine probiotics.

Carboxymethyl cellulose; Cellulolytic bacterium; Fermentation conditions; Screening and identification; Tibetan pig

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