Background: Chlorophytum borivilianum
is a rare medicinal plant originally distributed throughout the forest of
India. The tubers of C. borivilianum
are used as an aphrodisiac and impotence supplement. The propagation of
is possible through seeds and tubers, but conventional methods may take several months.
Hence in vitro
technique of shoot regeneration could be an efficient alternative means of propagating the
species. Latest study reported microtuberization of C. borivilianum
but there is no sufficient study on a rapid
method for shoot multiplication and elongation.
Young shoot buds of C. borivilianum
were cultured on MS medium containing 6-benzylaminopurine
(BAP) and Kinetin (Kn), both at 0, 8.88, 17.8 and 26.6 μM, either individually or in combinations. Proliferated
shoots were subcultured on fresh medium of the same constituents on week 3 of culture for further shoot
multiplication and elongation. BAP alone (8.88–26.6 μM) was significantly effective on shoot multiplication,
while Kn alone (8.88–26.6 μM) was significantly effective on shoot elongation compared to the control
containing MS basal medium without any plant growth regulator. However, combination of both cytokinins
stimulated an interaction producing higher shoot number and shoot length compared to their individual
The most suitable combination was 8.88 μM BAP + 8.88 μM Kn, reaching a mean shoot number of
10.83 and shoot length of 6.85 cm.