Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional
regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR
salt stress tolerance. The contexts of the studywas to analysis the sequence characterization of banana ASR1
, and identify
its expression patterns and function under salt stress using quantitative real-time PCR (qPCR) and overexpression in
. The purpose was to evaluate the role of banana ASR1
to salt stress tolerance employed by plants.
A full-length cDNA isolated from banana fruitwas named MaASR1
, and it had a 432 bp open reading frame (ORF)
encoding 143 amino acids. MaASR1
was preferential expression in roots and leaves compared to low expression in fruits,
rhizomes and flowers. Under salt stress, the expression of MaASR1
quickly increased and highest expression level was
detected in roots and leaves at 4 h, and then gradually decreased. These results suggested that MaASR1
induced under salt stress. MaASR1 protein was localized in the nucleus and plasma membrane. MaASR1
transformed to Arabidopsis
and verified by southern and northern analysis, transgenic lines L14 and L38 integrated one
and two copies of MaASR1
, respectively, while overexpression in transgenic lines provided evidence for the role of
to salt stress tolerance.
This study demonstrated that overexpression of MaASR1
confers salt stress tolerance by
reducing the expression of ABA/stress-responsive genes, but does not affect the expression of the ABA-independent
pathway and biosynthesis pathway genes.