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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 1, 2015, pp. 16-19
Bioline Code: ej15004
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 1, 2015, pp. 16-19

 en Fibrinolytic protease production by new Streptomyces check for this species in other resources sp. DPUA 1576 from Amazon lichens
Silva, Germana M.M.; Bezerra, Raquel P.; Teixeira, José A.; Porto, Tatiana S.; Lima-Filho, José L. & Porto, Ana Lúcia F.


Background: Streptomyces check for this species in other resources sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations.
Results: The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm2 was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions.
Conclusions: These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production.

Actinomycetes; Factorial design; Glucose; Microbial enzymes; Soybean flour

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