PPARγ, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development|
Xu, Xiaochun; Wei, Xuan; Yang, Yuxin; Niu, Wenzhi; Kou, Qifang; Wang, Xiaolong & Chen, Yulin
Background: The objective of this study was to investigate proliferator-activated receptor (PPARγ), fatty acid
synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep.
Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months) were selected, and their tail
measurements including length (L), width (W) and girth (G) were recorded. The mRNA and protein
expressions of PPARγ, FAS and HSL were examined by quantitative real-time polymerase chain reaction (PCR)
Results: The tail measurements increased with age.We observed no significant differences (P > 0.05) of PPARγ
mRNA expression between ages 9 and 15 months, and between 12 and 15 months; FAS mRNA expression
levels at each developmental stage were observed significantly in Tan sheep (P < 0.05); HSL mRNA expression
with no significant differences were only observed between 6 and 15 months (P > 0.05). Significant differences
(P < 0.05) of PPARγ, FAS and HSL protein expressions at each developmental stage were observed in Tan sheep.
Conclusion: We observed that the mRNA expression patterns of PPARγ and FAS decreased first before they
increased again and then this process repeated. Conversely, the mRNA expression patterns of HSL increased
first before they decreased and then this process repeated. The protein expression patterns of PPARγ and FAS
decreased first before they increased again and then this process repeated. Conversely, the protein expression
pattern of HSL increased first before it decreased again and then increased again.
Fat-tailed sheep; mRNA transcription; Protein expression; Transcription factor and enzyme genes