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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 2, 2015, pp. 134-137
Bioline Code: ej15023
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 2, 2015, pp. 134-137

 en Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
García-Baldenegro, Claudia Vanessa; Vargas-Arispuro, Irasema; Islas-Osuna, María; Rivera-Domínguez, Marisela; Aispuro-Hernández, Emmanuel & Martínez-Téllez, Miguel Ángel

Abstract

Background: Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the total RNA quality of dormant grapevine buds ( Vitis vinifera check for this species in other resources L. cv. ‘Flame Seedless’) of freeze-dried samples stored at room temperature conditions was evaluated and compared to that of cryopreserved (-80°C) grapevine buds.
Results: Good yield and quality of RNA were obtained from freeze-dried dormant buds stored at room temperature for 0, 3 and 6 weeks after they were lyophilized. Further experiments confirmed that the extracted total RNA could be used for actin and β-tubulin PCR gene amplification.
Conclusion: High-quality RNA that is useful for downstream applications was obtained from freeze-dried dormant grapevine bud tissue, similarly to the RNA obtained from cryopreserved dormant grapevine buds.

Keywords
Freeze-dried; RNA integrity number; RT-qPCR; Vitis vinifera L; Woody tissue

 
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