search
for
 About Bioline  All Journals  Testimonials  Membership  News


Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 3, 2015, pp. 148-153
Bioline Code: ej15026
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 3, 2015, pp. 148-153

 en Study on the relationship between intracellular metabolites and astaxanthin accumulation during Phaffia rhodozyma check for this species in other resources fermentation
Xiao, Anfeng; Jiang, Xinglong; Ni, Hui; Yang, Qiuming & Cai, Huinong

Abstract

Background: To study the relationship between intracellular anabolismand astaxanthin production, the influence of intracellular protein and fatty acids on astaxanthin production by four mutant Phaffia rhodozyma check for this species in other resources strains and their variations was investigated in this research.
Results: First, the content of astaxanthin in cells showed a reverse fluctuation in contrast to that of protein during the whole fermentation process. Moreover, compared with the three other strains, the astaxanthin-overproducing mutant strain of the yeast P. rhodozyma, called JMU-MVP14, had the highest specific productivity of astaxanthin as 6.8 mg/g, whereas its intracellular protein and fatty acid contents were the lowest. In addition, as a kind of sugar metabolic product, ethanol was only produced by P. rhodozyma JMU-VDL668 and JMU-7B12 during fermentation.
Conclusions: The results indicated that the accumulation of ethanol, intracellular protein, and fatty acids had competition effects on astaxanthin synthesis. This condition may explain why the P. rhodozyma strains JMU-VDL668 and JMU-7B12 achieved relatively lower astaxanthin production (1.7 and 1.2 mg/L) than the other two strains JMU-MVP14 and JMU-17W(20.4 and 3.9 mg/L).

Keywords
Astaxanthin; Ethanol; Fatty acids; Phaffia rhodozyma; Protein

 
© Copyright 2015 - Electronic Journal of Biotechnology
Alternative site location: http://www.ejbiotechnology.info

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil