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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 3, 2015, pp. 169-174
Bioline Code: ej15029
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 3, 2015, pp. 169-174

 en Molecular cloning and antibacterial activity of hepcidin from Chinese rare minnow ( Gobiocypris rarus check for this species in other resources )
Ke, Fei; Wang, Yun; Yang, Chuan-Shun & Xu, Chen

Abstract

Background: Hepcidins, a kind of cysteine-rich antimicrobial peptides, play important roles in host immunological processes and iron regulation, which have been identified from several fish species. The rare minnow ( Gobiocypris rarus check for this species in other resources ), an endemic cyprinid fish in China, has been used extensively as model animal in laboratory. However, little is known about its hepcidin. Here, we report the cloning and characterization of a hepcidin gene from the liver of Chinese rare minnow.
Results: The full-length cDNA of rare minnow hepcidin is 662 bp, which contains an ORF of 273 bp encoding a prepropeptide of 90 amino acid residues. The predicted prepropeptide contains three domains: a signal peptide of 24 amino acids, a prodomain of 41 amino acids, and a mature peptide of 25 amino acids. Sequence alignment showed eight conserved cysteine residues in the mature peptide, which formed four disulfide bonds in spatial structure. The deduced structure of mature peptide showed a high degree of homology to the human hepcidin. Phylogenetic analysis showed that it had a close relationship with zebrafish hepcidin, and clustered in a clade with these from Cyprinidae. Synthetic peptide of rare minnow hepcidin could inhibit the growth of Gram positive bacterium Staphylococcus aureus check for this species in other resources and Gram negative bacteria Escherichia coli check for this species in other resources and Aeromonas hydrophila check for this species in other resources .
Conclusion: These results suggested that rare minnow hepcidin had typical structure of hepcidins and antibacterial activity. It could participate in innate immune response as an antibacterial agent and be used as antibiotic substance.

Keywords
Cysteine residues; Phylogenetic analysis; Rare minnow hepcidin; Synthetic peptide

 
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