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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 3, 2015, pp. 188-195
Bioline Code: ej15032
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 3, 2015, pp. 188-195

 en Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum check for this species in other resources (Imazeki) and characterization of their immunomodulatory properties
Ubaidillah, Nik Hafizah Nik; Abdullah, Noorlidah & Sabaratnama, Vikineswary

Abstract

Background: The role of polysaccharides isolated from the Ganoderma check for this species in other resources species of fungi in innate immunity has recently become a topic of research. Although some work has been conducted concerning Ganoderma lucidum check for this species in other resources , the characteristics of polysaccharides isolated from Ganoderma neojaponicum check for this species in other resources (Imazeki) as immunomodulatory agents are largely unknown. The aims for this study were to isolate and characterize the intracellular polysaccharides (IPSs) and extracellular polysaccharides (EPSs) of G. neojaponicum from STR reactor.
Results: The production of EPS and IPS was optimized on day 4 of the cultivation time in 2 L STR reactor based on the amount of biomass yield, total carbohydrate, β-glucan and α-glucan content. Further analysis, both the EPSs and IPSs showed the enhancement on proliferation and increment of phagocytosis activities of macrophage (RAW264.7) cell lines. Using an oral toxicity test, we also observed that 2000 mg/kg body weight/day dosage of dried G. neojaponicum mycelium does not cause any significant toxic effects on Sprague–Dawley rats in 14 d of administration.
Conclusion: The findings of this study indicate that the IPSs and EPSs of G. neojaponicum have the potential to be used as immunomodulating agents to stimulate the innate immune system for fighting infectious diseases. The polysaccharides from G. neojaponicum have to be further commercially explored as an alternative for medicinal Ganoderma variety of G. lucidum production.

Keywords
β-Glucan; Acute oral toxicity; Macrophage cell line; Phagocytosis; Stirred tank reactor; Total carbohydrate

 
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