mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep|
Xu, Xiaochun; Wei, Xuan; Yang, Yuxin; Niu, Wenzhi; Kou, Qifang; Wang, Xiaolong & Chen, Yulin
Background: The objective of this study was to compare the level differences of mRNA transcription and protein
expression of PPARγ, FAS and HSL in different parts of the carcass in different tail-type sheep. Six Tan sheep and
six Shaanbei fine-wool sheep aged 9monthswere slaughtered and sampleswere collected fromthe tail adipose,
subcutaneous adipose, and longissimus dorsimuscle. The levels ofmRNA transcription and protein expression of
the target genes in these tissues were determined by real-time quantitative PCR and western blot analyses.
Results: The results showed that PPARγ, FAS, and HSL were expressed with spatial differences in tail adipose,
subcutaneous adipose and longissimus dorsi muscle of Tan sheep and Shaanbei fine-wool sheep. Differences
were also observed between the two breeds. The mRNA transcription levels of these genes were somewhat
consistent with their protein expression levels.
Conclusion: The present results indicated that PPARγ, FAS and HSL are correlated with fat deposition, especially
for the regulating of adipose deposition in intramuscular fat, and that the mRNA expression patterns are
similar to the protein expression patterns. The mechanism requires clarification in further studies.
Genes; Difference; Tissues; Ovis