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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 4, 2015, pp. 281-285
Bioline Code: ej15045
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 4, 2015, pp. 281-285

 en Fermentation optimization of maltose-binding protein fused to neutrophil-activating protein from Escherichia coli check for this species in other resources TB1
Lu, Jike; Song, Qi; Ji, Zhenyu; Liu, Xin; Wang, Ting & Kang, Qiaozhen

Abstract

Background: The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori check for this species in other resources were optimized from Escherichia coli check for this species in other resources TB1 with varying medium, inoculum age and size, time, inducer, pH and temperature in batch fermentation.
Results: It was revealed that the optimal conditions for the production of rMBP-NAP in shake flask were as follows: M9 medium (with 3% yeast extract powder added), inoculum age of 19 h, inoculum size of 6%, initial pH of 6.6, temperature of 37°C, and 0.7 mmoL/L IPTG inducted 21 h in a 50 mL/250 mL shake flask. The recombinant protein yield was increased from 59 to 592 mg/L after optimization. Fermentation process conducted in a 10 L fermenter with similar conditions could get 30 g/L wet cell and 1.738 g/L soluble protein with the rMBP-NAP expression level of 11.9%.
Conclusion: The results improve the expression level of rMBP-NAP, and it is expected that these optimized conditions can be well applied for large scale production of rMBP-NAP.

Keywords
Escherichia coli; Fermentation; Optimization; Recombinant protein

 
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