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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 4, 2015, pp. 295-301
Bioline Code: ej15048
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 4, 2015, pp. 295-301

 en Phenolic compound production and biological activities from in vitro regenerated plants of gherkin ( Cucumis anguria check for this species in other resources L.)
Thiruvengadam, Muthu & Chung, Ill-Min

Abstract

Background: The effect of polyamines (PAs) along with cytokinins (TDZ and BAP) and auxin (IBA)was induced by the multiple shoot regeneration from leaf explants of gherkin ( Cucumis anguria check for this species in other resources L.). The polyphenolic content, antioxidant and antibacterial potential were studied from in vitro regenerated and in vivo plants.
Results: Murashige and Skoog (MS) medium supplemented with 3% sucrose containing a combination of 3.0 μM TDZ, 1.0 μM IBA and 75 μM spermidine induced maximum number of shoots (45 shoots per explant) was achieved. Regenerated shoots elongated in shoot elongation medium containing 1.5 μM GA3 and 50 μM spermine. The well-developed shoots were transferred to root induction medium containing 1.0 μM IBA and 50 μM putrescine. Rooted plants were hardened and successfully established in soil with a 95% survival rate. Twenty-five phenolic compounds were identified by ultra-performance liquid chromatography (UPLC) analysis The individual polyphenolic compounds, total phenolic and flavonoid contents, antioxidant and antibacterial potential were significantly higher with in vitro regenerated plants than in vivo plants.
Conclusions: Plant growth regulators (PGRs) and PAs had a significant effect on in vitro plant regeneration and also a biochemical accumulation of flavonols, hydroxybenzoic and hydroxycinnamic acid derivatives in C. anguria. Due to these metabolic variations, the antioxidant and antibacterial activities were increased with in vitro regenerated plants than in vivo plants. This is the first report describing the production of phenolic compounds and biological activities from in vitro and in vivo regenerated plants of C. anguria.

Keywords
Biological activities; Cucumis anguria; Multiple shoot induction; Plant growth regulators; Polyamines; Polyphenolic content

 
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