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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 4, 2015, pp. 327-332
Bioline Code: ej15053
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 4, 2015, pp. 327-332

 en Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus check for this species in other resources
Hernández, Christian A.; Sandoval, Norma; Mallerman, Julieta; García-Pérez, José Antonio; Farnet, Anne-Marie; Perraud-Gaime, Isabelle & Alarcón, Enrique

Abstract

Background: Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiencywith some fungi. In this study,we hypothesized that ethanol laccase induced in Pycnoporus sanguineus check for this species in other resources depends on nitrogen nutriment conditions. To prove this,we assessed laccase production in submerged cultures of P. sanguineus,with different nitrogen concentrations andwith, orwithout ethanol added in a factorial designed experiment.
Results: In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and ethanol (3 g/L) induced the highest laccase activity (31.01 ± 4.9 U/L), while the treatments with urea reached the lowest activity (less than 1.6 U/L). Ethanol had positive and synergic effects on laccase production, in accordance with the surface response model, as long as simple nitrogen sources (urea) were not available.
Conclusions: We suggest that laccase in P. sanguineus is regulated by a catabolic nitrogen repressionmechanism; laccase activity is strongly inhibited by urea used as nitrogen source and it decreases when the amount of urea increases; contrarily, a synergic positive effect was observed between yeast extract and ethanol on laccase production.

Keywords
Enzyme production; Fungal metabolism; Phenoloxidases; Urea; Yeast extract

 
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