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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 6, 2015, pp. 393-398
Bioline Code: ej15067
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 6, 2015, pp. 393-398

 en Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa check for this species in other resources
Mestanza, Camilo; Riegel, Ricardo; Silva, Herman & Vásquez, Santiago C.

Abstract

Background: Currently, the technology called Clearfield® is used in the development of crops resistant to herbicides that inhibit the enzyme acetohydroxy acid synthase (AHAS, EC 2.2.1.6). AHAS is the first enzyme of the biosynthetic pathway that produces the branched-chain of the essential amino acids valine, leucine, and isoleucine. Therefore, multiple copies of the AHAS gene might be of interest for breeding programs targeting herbicide resistance. In this work, the characterization of the AHAS gene was accomplished for the Chenopodium quinoa check for this species in other resources Regalona-Baer cultivar. Cloning, sequencing, and Southern blotting were conducted to determine the number of gene copies.
Results: The presence of multiple copies of the AHAS gene as has been shownpreviously in several other species is described. Six copies of the AHAS gene were confirmed with Southern blot analyses. CqHAS1 and CqAHAS2 variants showed the highest homology with AHAS mRNA sequences found in the NR Database. A third copy, CqAHAS3, shared similar fragments with both CqAHAS1 and CqAHAS2, suggesting duplication through homeologous chromosomes pairing.
Conclusions: The presence of multiple copies of the gene AHAS shows that gene duplication is a common feature in polyploid species during evolution. In addition, to our knowledge, this is the first report of the interaction of sub-genomes in quinoa.

Keywords
Acetohydroxyacid synthase; Acetolactate synthase; Chenopodium quinoa; Homeologous pairing; Gene duplication

 
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