Background:
An endophytic fungus lives within a healthy plant during certain stages of, or throughout, its life
cycle. Endophytic fungi do not always cause plant disease, and they include fungi that yield different effects,
including mutual benefit, and neutral and pathogenic effects. Endophytic fungi promote plant growth, improve
the host plant's resistance to biotic and abiotic stresses, and can produce the same or similar biologically active
substances as the host. Thus, endophytic fungal products have important implications in drug development.
Result:
Among the numerous endophytic fungi, we identified two strains, L10Q37 and LQ2F02, that have
anti-acetylcholinesterase activity, but the active compound was not huperzine A. The aim of this study was to
investigate the anti-acetylcholinesterase activity of secondary metabolites isolated from the endophytic fungi
of
Huperzia serrata
. Microbial cultivation and fermentation were used to obtain secondary metabolites. Active
components were then extracted from the secondary metabolites, and their activities were tracked. Two
compounds that were isolated from endophytic fungi of
H. serrata were identified and had acetylcholine
inhibitory activities. In conclusion, endophytic fungal strains were found in
H. serrata that had the same
anti-acetylcholinesterase activity.
Conclusion:
We isolated 4 compounds from the endophytic fungus L10Q37, among them S1 and S3 are new
compounds. 6 compounds were isolated from LQ2F02, all 6 compounds are new compounds. After tested anti
acetylcholinesterase activity, S5 has the best activity. Other compounds' anti acetylcholinesterase activity was
not better compared with huperzine A.
