The classification of diversity in germplasm collections is important for plant breeding. The
repetitive element palindromic-polymerase chain reaction (rep-PCR) technique was used to investigate
inter-specific diversity within 17 species from the Euphorbiaceae family using REP and BOX primers.
The agglomerative cluster analysiswas used to evaluate the scoring data. BOX and REP gave amplification
with polymorphism of 98.84% and 100% respectively. REP marker demarcated between the subgenus peltatae.
Both markers confirmed Jatropha tanjorensis
as a natural hybrid between Jatropha gossypifolia
and Jatropha curcas
. Five random sequences from the rep-PCR gels were chosen, cloned and sequenced. The blast results
demonstrated that the amplified products were from the mitochondrial genomes.
The rep-PCR molecular tool can be used to characterize diversity in plants as they are suitable for
distinguishing eukaryotic genomes effectively.