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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 6, 2015, pp. 412-417
Bioline Code: ej15070
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 6, 2015, pp. 412-417

 en Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
Rampadarath, Sillma; Puchooa, Daneshwar & Bal, Subhasisa

Abstract

Background: The classification of diversity in germplasm collections is important for plant breeding. The repetitive element palindromic-polymerase chain reaction (rep-PCR) technique was used to investigate inter-specific diversity within 17 species from the Euphorbiaceae family using REP and BOX primers.
Results: The agglomerative cluster analysiswas used to evaluate the scoring data. BOX and REP gave amplification with polymorphism of 98.84% and 100% respectively. REP marker demarcated between the subgenus peltatae. Both markers confirmed Jatropha tanjorensis check for this species in other resources as a natural hybrid between Jatropha gossypifolia check for this species in other resources and Jatropha curcas check for this species in other resources . Five random sequences from the rep-PCR gels were chosen, cloned and sequenced. The blast results demonstrated that the amplified products were from the mitochondrial genomes.
Conclusion: The rep-PCR molecular tool can be used to characterize diversity in plants as they are suitable for distinguishing eukaryotic genomes effectively.

Keywords
Euphorbiaceae; Genetic diversity; Jatropha; Mitochondrial genome; rep-PCR

 
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