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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 6, 2015, pp. 439-444
Bioline Code: ej15074
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 6, 2015, pp. 439-444

 en Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi check for this species in other resources
Rocha, Carla Maria Lourdes; Vellicce, Gabriel Ricardo; García, María Gabriela; Pardo, Esteban Mariano; Racedo, Josefina; Perera, María Francisca; de Lucía, Adrian; Gilli, Javier; Bogado, Noelia; Bonnecarrère, Victoria; German, Silvia; Marcelino, Francismar; Ledesma, Fernando; Reznikov, Sebastián; Ploper, Leonardo Daniel; Welin, Bjorn & Castagnaro, Atilio Pedro

Abstract

Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi check for this species in other resources Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique.
Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled.
Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.

Keywords
Asian soybean rust; Genetic variation; Glycine max; Molecular markers

 
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