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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 6, 2015, pp. 471-479
Bioline Code: ej15078
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 6, 2015, pp. 471-479

 en Application of bacterial and yeast biosurfactants for enhanced removal and biodegradation of motor oil from contaminated sand
Chaprão, Marcos J.; Ferreira, Isabela N. S.; Correa, Priscilla F.; Rufino, Raquel D.; Luna, Juliana M.; Silva, Elias J. & Sarubbo, Leonie A.


Background: This study investigated the potential application of two biosurfactants for enhanced removal capability and biodegradation of motor oil contaminated sand under laboratory conditions. The biosurfactants were produced by the yeast Candida sphaerica check for this species in other resources and by the bacterium Bacillus check for this species in other resources sp. cultivated in low-cost substrates. The ability of removing motor oil from soil by the two biosurfactants was identified and compared with that of the synthetic surfactants Tween 80 and Triton X-100.
Results: Both crude and isolated biosurfactants showed excellent effectiveness on motor oil removal from contaminated sand under kinetic conditions (70–90%), while the synthetic surfactants removed between 55 and 80% of the oil. A contact time of 5–10 min under agitation seemed to be enough for oil removal with the biosurfactants and synthetic surfactants tested. The crude and the isolated biosurfactant from C. sphaerica were able to remove high percentages of motor oil from packed columns (around 90%) when compared to the biosurfactant from Bacillus sp. (40%). For the degradation experiments conducted in motor oil contaminated sand enriched with sugar cane molasses, however, oil degradation reached almost 100% after 90 d in the presence of Bacillus sp. cells, while the percentage of oil degradation did not exceed 50% in the presence of C. sphaerica. The presence of the biosurfactants increased the degradation rate in 10–20%, especially during the first 45 d, indicating that biosurfactants acted as efficient enhancers for hydrocarbon biodegradation.
Conclusions: The results indicated the biosurfactants enhancing capability on both removal and rate of motor oil biodegradation in soil systems.

Bacillus sp.; Bioremediation; Candida sphaerica; Petroleum; Sand-packed column

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