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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 19, No. 1, 2016, pp. 15-22
Bioline Code: ej16003
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 19, No. 1, 2016, pp. 15-22

 en Over-expression of CYP78A98, a cytochrome P450 gene from Jatropha curcas check for this species in other resources L., increases seed size of transgenic tobacco
Tian, Yinshuai; Zhang, Min; Hu, Xiaole; Wang, Linghui; Dai, Jiao; Xu, Ying & Chen, Fang

Abstract

Background: Jatropha curcas check for this species in other resources L. (further referred to as Jatropha), as a rapidly emerging biofuel crop, has attracted worldwide interest. However, Jatropha is still an undomesticated plant, the true potential of this shrub has not yet been fully realized. To explore the potential of Jatropha, breeding and domestication are needed. Seed size is one of themost important traits of seed yield and has been selected since the beginning of agriculture. Increasing the seed size is amain goal of Jatropha domestication for increasing the seed yield, but the genetic regulation of seed size in Jatropha has not been fully understood.
Results: We cloned CYP78A98 gene from Jatropha, a homologue of CYP78A5 in Arabidopsis. We found that CYP78A98 was highly expressed in male flower, female flower, stem apex, leaf and developing seed. However, its transcripts were hardly detected in root and stem. CYP78A98 protein localized in endoplasmic reticulum (ER) and the hydrophobic domain at the N-terminus was essential for the correct protein localization. Furthermore, INNER NO OUTER promoter (pINO) drove specific overexpression of CYP78A98 in transgenic tobacco seeds resulted in increased seed size andweight, aswell as improved seed protein and fatty acid content.
Conclusions: The results indicated that CYP78A98 played a role in Jatropha seed size control. This may help us to better understand the genetic regulation of Jatropha seed development, and accelerate the breeding progress of Jatropha.

Keywords
Biodiesel; Breeding; CYP78A5; Jatropha

 
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