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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 19, No. 1, 2016, pp. 33-40
Bioline Code: ej16006
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 19, No. 1, 2016, pp. 33-40

 en Three new shuttle vectors for heterologous expression in Zymomonas mobilis check for this species in other resources
Cao, Qinghua; Li, Tao; Shao, Huanhuan; Tan, Xuemei & Zhang, Yizheng

Abstract

Background: Zymomonas mobilis check for this species in other resources , as a novel platform for bio-ethanol production, has been attractedmore attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium.
Results: Three shuttle vectors (pSUZM1, pSUZM2 and pSUZM3)were first constructedwith the origins of replication from the chromosome and two native plasmids (pZZM401 and pZZM402) of Z. mobilis ZM4, respectively. The three shuttle vectorswere stable in Z. mobilis ZM4 and have 3, 32 and 27 copies, respectively. The promoter Ppdc (a), from the pyruvate decarboxylase gene,was cloned into the shuttle vectors, generating the expression vectors pSUZM1(2, 3)a. The codon-optimized glucoamylase gene from Aspergillus awamori check for this species in other resources combined with the signal peptide sequence from the alkaline phosphatase gene of Z. mobilis was cloned into pSUZM1(2, 3)a, resulting in the plasmids pSUZM1a-GA, pSUZM2a-GA and pSUZM3a-GA, respectively. After transforming these plasmids into Z. mobilis ZM4, the host was endowed with glucoamylase activity for starch hydrolysis. Both pSUZM2a-GA and pSUZM3a-GA were more efficient at producing glucoamylase than pSUZM1a-GA.
Conclusions: These results indicated that these expression vectors are useful tools for gene expression in Z. mobilis and this could provide a solid foundation for further studies of heterologous gene expression in Z. mobilis.

Keywords
Expression vector; Gene expression; Glucoamylase; Zymomonas mobilis

 
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