Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida   BET001 and modeling of its fermentation in shake flasks

Background: The paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida

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BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling.
Results: The biomass production, fatty acids consumption andmcl-PHA production were found favorable when the strain was cultured inmineral medium at pH 6–7, 28°C, aeration surface-to-volume ratio of 0.4 × 10⁶m^-1, 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated components as described by Luedeking–Piret kinetic model.
Conclusion: The findings of this study provided add to the literature on key variables in for achieving good microbial growth andmcl-PHA production in shake flasks culture. In addition, suitable kineticmodel to describe cultivation in this system was also presented.

Keywords
Biopolymer; Fermentation; Medium-chain-length PHA; Palm kernel oil; Pseudomonas putida