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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 19, No. 1, 2016, pp. 56-60
Bioline Code: ej16009
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 19, No. 1, 2016, pp. 56-60

 en Effect of malate on docosahexaenoic acid production from Schizochytrium check for this species in other resources sp. B4D1
Zhang, Yong; Min, Qingsong; Xu, Jian; Zhang, Ke; Chen, Shulin; Wang, Haijun & Li, Demao


Background: Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium check for this species in other resources species during fermentation.
Results: DHA production increased by 47% and reached 5.51 g/L when 4 g malate/Lwas added during the rapid lipid accumulation stage in shake-flasks culture. Inducing effects ofmalate was further investigated through the analysis of three kinetic parameters, including specific cell growthrate (μ), specific glucose consumption rate (qGlu) andDHA formation rate (qDHA). DHA concentration was enhanced through a novel fed-batch strategy to amaximumvalue of 30.7 g/L, giving a yield of 0.103 g DHA/g glucose and a productivity of 284 mg L-1 h-1.
Conclusion: A novel malate feeding strategy was developed that enhanced DHA yield and productivity of Schizochytrium species which may offer a desirable method for industrial applications.

DHA; Malate; NADPH; Schizochytrium sp.

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