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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 21, No. 1, 2016, pp. 77-81
Bioline Code: ej16027
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 21, No. 1, 2016, pp. 77-81

 en DNA demethylation during Chrysanthemum check for this species in other resources floral transition following short-day treatment
Li, Zhongai; Li, Jie; Liu, Yanhua & Wang, Zicheng

Abstract

Background: Analytical techniques such as methylation-sensitive amplification polymorphism and high-performance liquid chromatography were used to detect variation in DNA methylation of mature Chrysanthemum check for this species in other resources leaves during the floral transition induced by short-day (SD) treatment.
Results: For both early- and late-flowering cultivars, the time from the date of planting to the appearance of the capitulum bud and early blooming were significantly shorter than those of the control. The capitulum development of the early-flowering cultivar was significantly accelerated compared to the control, unlike the late-flowering cultivar. The DNA methylation percentage of leaves was significantly altered during flower development. For the early-flowering cultivar, DNA methylation was 42.2–51.3% before the capitulum bud appeared and 30.5–44.5% after. The respective DNA methylation percentages for the late-flowering cultivar were 43.5–56% and 37.2–44.9%.
Conclusions: The DNA methylation percentage of Chrysanthemum leaves decreased significantly during floral development. The decline in DNA methylation was elevated in the early-flowering cultivar compared with the late-flowering cultivar.

Keywords
Chrysanthemum; DNA methylation; Floral transition; Short day

 
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