species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psb
H, were used to authenticate and analyze the diversity of five Memecylon
species collected from Western Ghats of India.
Phylogenetic analysis clearly distinguished Memecylon malabaricum
from Memecylon wightii
and Memecylon umbellatum
from Memecylon edule
and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon
species were distinctly separated into three different clades. M. malabaricum
and M. wightii
grouped together and M. umbellatum
, M. edule
and Memecylon talbotianum
grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar.
Data from the present study reveals that chloroplast psb
H region could be used as a potential candidate region for identifying Memecylon
species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker.