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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 24, No. 1, 2016, pp. 1-8
Bioline Code: ej16052
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 24, No. 1, 2016, pp. 1-8

 en Identification and genetic diversity analysis of Memecylon check for this species in other resources species using ISSR, RAPD and Gene-Based DNA barcoding tools
Ramasetty, Bharathi Tumkur; Bajpe, Shrisha Naik; Kadappa, Sampath Kumara Kigga; Saini, Ramesh Kumar; Basavaraju, Shashibhushan Nittur; Ramachandra, Kini Kukkundoor & Sripathy, Prakash Harishchandra

Abstract

Background: Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-atpH, were used to authenticate and analyze the diversity of five Memecylon species collected from Western Ghats of India.
Results: Phylogenetic analysis clearly distinguished Memecylon malabaricum check for this species in other resources from Memecylon wightii check for this species in other resources and Memecylon umbellatum check for this species in other resources from Memecylon edule check for this species in other resources and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon species were distinctly separated into three different clades. M. malabaricum and M. wightii grouped together and M. umbellatum, M. edule and Memecylon talbotianum check for this species in other resources grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar.
Conclusion: Data from the present study reveals that chloroplast psbA-trnH region could be used as a potential candidate region for identifying Memecylon species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker.

Keywords
atpF-atpH; Memecylon species; ndh; psbA-trnH; rpoC1; 5s; PCR based techniques; Molecular phylogenetic analysis; Molecular phylogenesis; DNA markers

 
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