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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 24, No. 1, 2016, pp. 12-20
Bioline Code: ej16054
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 24, No. 1, 2016, pp. 12-20

 en Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella check for this species in other resources sp. Ponipodef12 after treatments with metal ions
Luo, Haiyu; Xu, Dan; Xie, Rushan; Zhang, Xuping; Wang, Jian; Dong, Xuejiao; Lai, Daowan; Zhou, Ligang & Liu, Yang

Abstract

Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e., Mn2+, Na+, Mg2+, Zn2+, Cu2+, Fe2+, Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated.
Results: Three most effective metal ions (Zn2+, Cu2+ and Mg2+) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2+, Cu2+ and Mg2+ were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+, Cu2+ and Mg2+ on the production of botrallin and TMC-264 by employing statistical method based on the central composite design (CCD) and response surface methodology (RSM) were evaluated, and two quadratic predictive models were developed for botrallin and TMC-264 production. The yields of botrallin and TMC-264, which were predicted as 144.12 mg/L and 36.04 mg/L respectively, were validated to be 146.51 mg/L and 36.63 mg/L accordingly with the optimum concentrations of Zn2+ at 0.81 mmol/L, Cu2+ at 0.20 mmol/L, and Mg2+ at 0.13 mmol/L in medium.
Conclusion: The results indicated that the enhancement of botrallin and TMC-264 accumulation in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 by the metal ions and their combination should be an effective strategy.

Keywords
Combination effects; Production; Microbial biotechnology; Process biotechnology; Bioactivities; Central composite design; Response surface methodology

 
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