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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 24, No. 1, 2016, pp. 26-31
Bioline Code: ej16056
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 24, No. 1, 2016, pp. 26-31

 en Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii check for this species in other resources
Pan, Long; Fang, Ya-kun; Zhou, Pei; Jin, Kui-qi; Wang, Gang & Liu, Yu-peng

Abstract

Background: The effect of diverse oxygen transfer coefficient on the L-erythrulose production from meso-erythritol by a newly isolated strain, Gluconobacter kondonii CGMCC8391 was investigated. In order to elucidate the effects of volumetric mass transfer coefficient (kLa) on the fermentations, baffled and unbaffled flask cultures, and fed-batch cultures were developed in present work.
Results: With the increase of the kLa value in the fed-batch culture, L-erythrulose concentration, productivity and yield were significantly improved, while cell growth was not the best in the high kLa. Thus, a two-stage oxygen supply control strategy was proposed, aimed at achieving high concentration and high productivity of L-erythrulose. During the first 12 h, kLa was controlled at 40.28 h-1 to obtain high value for cell growth, subsequently kLa was controlled at 86.31 h-1 to allow for high L-erythrulose accumulation.
Conclusions: Under optimal conditions, the L-erythrulose concentration, productivity, yield and DCW reached 207.9 ± 7.78 g/L, 6.50 g/L/h, 0.94 g/g, 2.68 ± 0.17 g/L, respectively. At the end of fermentation, the L-erythrulose concentration and productivity were higher than those in the previous similar reports.

Keywords
kLa; Industrial biotechnology; Process control; Production; Tanning agents; Tanning properties; Artificial tanning; Dihydroxypropanone (DHA); Production of L-erythrulose; Meso-erythritol; Biosynthesis of L-erythrulose

 
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