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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 25, No. 1, 2017, pp. 1-8
Bioline Code: ej17001
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 25, No. 1, 2017, pp. 1-8

 en Over-expression of dehydroascorbate reductase enhances oxidative stress tolerance in tobacco
Chang, Lingmo; Sun, Huan; Yang, Hua; Wang, Xuehua; Su, Zhizhe; Chen, Fang & Wei, Wei


Background: Ascorbic acid (Asc) is one of the most abundant antioxidants and it serves as amajor contributor to protect plants against oxidative damage. Plants use two enzymes that participate in the metabolic recycling of Asc. One of these two enzymes is dehydroascorbate reductase (DHAR). It directly regenerates Asc from its oxidized state and thus prevents Asc from being irreversibly hydrolyzed to 2, 3-diketogulonic acid. This study aimed to examine whether over-expression of DHAR leads to an enhanced oxidative stress tolerance in tobacco plants.
Results: In this study, we functionally characterized a novel JcDHAR gene from Jatropha curcas check for this species in other resources and found via quantitative RT-PCR analysis that JcDHAR can be induced with H2O2, salt and PEG stresses. The DHAR activities of transgenic tobacco plants increased from 2.0 to 5.3 fold compared to wild-type plants. As a result, the transgenic plants displayed enhanced tolerance to oxidative stress.
Conclusions: Our results indicate that JcDHAR expression can effectively enhance the tolerance to oxidative stress in plants.

Antioxidants; Ascorbic acid; DHAR; H2O2 stress; Jatropha curcas L.; Metabolic recycling of ascorbic acid; Oxidative damage; Quantitative RT-PCR; ROS; Salt stress; PEG stress

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