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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 25, No. 1, 2017, pp. 64-69
Bioline Code: ej17011
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 25, No. 1, 2017, pp. 64-69

 en Effect of miR-125b on dermal papilla cells of goat secondary hair follicle
Zhou, Guangxian; Yuan, Chao; He, Xiaolin; Kang, Danju; Wang, Xiaolong & Chen, Yulin

Abstract

Background: MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate various biological processes. miR-125b is a miRNA that has been reported to be critical for hair follicle (HF) morphogenesis and development. We identified that the expression of miR-125b varies during an individual hair cycle (anagen, catagen, and telogen) in the skin of cashmere goats. We constructed a gain model (by overexpressing miR-125b) and a loss model (by inhibiting endogenous miR-125b) based on dermal papilla cells (DPCs) to further investigate the role of miR-125b in HF cycle. In addition, we used a dual-luciferase system to highlight the predicated target genes of miR-125b.
Results: We found that miR-125b affects the expression of FGF5, IGF-1, SHH, TNF-α, MSX2, LEF-1, FGF7, NOGGIN, BMP2, BMP4, TGF-β1, and β-catenin. The dual-luciferase assay further validated a direct interaction between miR-125b and FGF5 and TNF-α.
Conclusion: miR-125b affects the expression levels of genes related to hair cycle and may also play a critical role in regulating the periodic development of HF.

Keywords
Anagen; Antagomir; Cashmere goats; Catagen; Dermal papilla cells; Hair cycle; Hair follicle development; Hair follicle morphogenesis; Luciferase assay; MicroRNAs; Recombinant adenovirus

 
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