Effect of miR-125b on dermal papilla cells of goat secondary hair follicle|
Zhou, Guangxian; Yuan, Chao; He, Xiaolin; Kang, Danju; Wang, Xiaolong & Chen, Yulin
Background: MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate various biological processes.
miR-125b is a miRNA that has been reported to be critical for hair follicle (HF) morphogenesis and
development. We identified that the expression of miR-125b varies during an individual hair cycle (anagen,
catagen, and telogen) in the skin of cashmere goats. We constructed a gain model (by overexpressing
miR-125b) and a loss model (by inhibiting endogenous miR-125b) based on dermal papilla cells (DPCs) to
further investigate the role of miR-125b in HF cycle. In addition, we used a dual-luciferase system to highlight
the predicated target genes of miR-125b.
Results: We found that miR-125b affects the expression of FGF5, IGF-1, SHH, TNF-α, MSX2, LEF-1, FGF7, NOGGIN,
BMP2, BMP4, TGF-β1, and β-catenin. The dual-luciferase assay further validated a direct interaction between
miR-125b and FGF5 and TNF-α.
Conclusion: miR-125b affects the expression levels of genes related to hair cycle and may also play a critical role in
regulating the periodic development of HF.
Anagen; Antagomir; Cashmere goats; Catagen; Dermal papilla cells; Hair cycle; Hair follicle development; Hair follicle morphogenesis; Luciferase assay; MicroRNAs; Recombinant adenovirus